The response of yeast vacuolar proteins: A novel rapid tool for Salmonella sp. screening.

Affiliation

Nguyen NT(1), Lee J(2), Woo SM(3), Kim YH(4), Min J(1)(2).
Author information:
(1)Department of Chemical Engineering, Jeonbuk National University, Deokjin-Gu Jeonju, Jeonbuk, South Korea.
(2)Graduate school of Semiconductor and Chemical Engineering, Chonbuk National University, Deokjin-Gu Jeonju, Jeonbuk, South Korea.
(3)Department of Food Science and Biotechnology, Shin Ansan University, Danwon-Gu, Ansan, South Korea.
(4)School of Biological Sciences, Chungbuk National University, Seowon-Gu, Cheongju, South Korea.

Abstract

Human health is recently affected by several factors in which food contamination is one of the most dangerous elements that damage directly on our bodies. In this study, we provided a novel approach for the rapid detection of Salmonella sp. at the molecular level using the response of Saccharomyces cerevisiae's vacuoles. First, an augmentation of vacuoles intensity was observed by confocal microscopy after treating Salmonella strains with yeast cells. Second, the vacuolar enzymes were isolated and then analyzed by two-dimensional electrophoresis for the screening of specific biomarkers. After that, various recombinant yeasts containing exclusive biomarkers were constructed by fusing these biomarkers with several fluorescent proteins. Finally, the recombinant strains showed the ability to detect Salmonella strains specifically by appropriate fluorescent signals from 20 CFU/mL after 15 Min of exposure.