Kim Y(1), Youn YS(2), Oh KT(3), Kim D(4), Lee ES(1)(5). Author information:
(1)Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro,
Bucheon-si, Gyeonggi-do 14662, Republic of Korea.
(2)School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu,
Suwon-si, Gyeonggi-do 16419, Republic of Korea.
(3)College of Pharmacy, Chung-Ang University, 221 Heukseok dong, Dongjak-gu,
Seoul 06974, Republic of Korea.
(4)Department of Pharmaceutical Sciences, College of Pharmacy, University of
Oklahoma Health Sciences Center, 1110 N Stonewall Ave, Oklahoma City, Oklahoma
73117, United States.
(5)Department of Biomedical-Chemical Engineering, The Catholic University of
Korea, 43 Jibong-ro, Bucheon-si, Gyeonggi-do 14662, Republic of Korea.
In this study, the strategy of transient generation of holes in the liposome surface has been shown to enable safe encapsulation of a high-molecular weight antibody (rituximab, Mw ∼140 kDa) within liposomes. These transient holes generated using our magnetoporation method allowed rituximab to safely enter the liposomes, and then the holes were plugged using hyaluronic acid grafted with 3-diethylaminopropylamine (DEAP). In the tumor microenvironment, the resulting liposomal rituximab was destabilized because of the ionization of the DEAP moiety at the acidic pH 6.5, resulting in extensive release of rituximab. Consequently, the rituximab released from the liposomes accumulated at high levels in tumors and bound to the CD20 receptors overexpressed on Burkitt lymphoma Ramos cells. This event led to significant enhancement in tumor cell ablation through rituximab-mediated complement-dependent cytotoxicity and Bcl-2 signaling inhibition-induced cell apoptosis.
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