Two Ecdysteroids Isolated from Micropropagated Lychnis flos-cuculi and the Biological Activity of Plant Material.

Affiliation

Maliński MP(1), Budzianowski J(1), Kikowska M(1), Derda M(2), Jaworska MM(3), Mlynarczyk DT(4), Szukalska M(5), Florek E(5), Thiem B(1).
Author information:
(1)Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland.
(2)Chair and Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, Poland.
(3)Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, 4 Święcickiego St., 60-781 Poznań, Poland.
(4)Chair and Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, 6 Grunwaldzka St., 60-780 Poznań, Poland.
(5)Laboratory of Environmental Research, Chair and Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, Poland.

Abstract

Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani. Additionally, a Microtox® acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC50 of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC50 of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25-2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of L. flos-cuculi in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from L. flos-cuculi micropropagated, ecdysteroid-rich plant material.