Shilpi Show, Krishna Chattopadhyay, Chetana Ghosal.
Brajadulal Chattopadhyay, A microbial protein-assisted silica ball comprising of silica-nanoparticles with plausible optical properties for multiple applications(2018)SDRP Journal of Nanotechnology & Material Science 1(1)
Background: Production of mesoporous silica nanoparticles and its conceivable applications in the fields of chromatography, surface polishing, catalysis and drug delivery etc. has gained momentum recently. We demonstrate here an efficient methodology for the amicable synthesis of silica balls consisting of mesoporous silica nanoparticles (SiO2-NPs) by using a secretary protein (bioremediase). The protein was isolated from a thermophilic non-pathogenic bacterium BKH1 (GenBank Accession No. FJ177512).
Methods: Silica ball was formed at ambient temperature by mixing the dissolved bacterial protein dropwise to an organic precursor tetra-ethyl-orthosilicate (TEOS) solution at neutral pH environment. Surface morphology and compositional studies of prepared silica ball were carried out by using High-Resolution Transmission Electron Microscope (HRTEM) and Field Emission Scanning Electron Microscope equipped with Energy Dispersive X-ray Analyzer (FESEM-EDX). Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD) studies were further carried out for auxiliary characterization to determine the nature of SiO2-NPs. Stability of the as prepared SiO2-NPs was determined by noting the Zeta potential (?). The dye degradation activity of the silica balls was noted against different dyes.
Results: Silica ball thus formed consisted of silica nanoparticles whose average dimensions were 20 ± 10 nm (n = 100). The size of the silica ball and also the sizes of the constituents’ nanoparticles depend on the protein concentration in the reaction mixture. The result of zeta potential implied the moderate stability of SiO2-NPs at neutral pH environment. The SiO2-NPs showed green fluorescence emission which might have feasibly applications in the field of biomedical imaging. The decolourizing effect of silica ball on various dyes is a cost effective phenomenon as it can be used repeatedly.
Conclusion: The protein-assisted silica balls preparation has a special consequence as it is an environmentally benign, lucrative and one pot synthesis approach which could be used repeatedly for various biomedical and chromatographic packing purposes.
Huo, Q., Feng, J., Schuth, F., Stucky, G. D. (1997). Preparation of Hard Mesoporous Silica Spheres. Chemistry of Material, 9(1): 14 ? 17.View Article
Ozin, G. A. (1992). Nanochemistry: Synthesis in diminishing dimensions. Advance Materials, 4: 612 ? 649.View Article
Gunter. B., Gru?n, M., Unger K. K., Matsumoto, A., Kazuo, T. (1998). Tailored syntheses of nanostructured silicas: Control of particle morphology, particle size and pore size. Supramolecular Science, 5: 253 ? 259. 00016-9View Article
Bao, X., Wei, X., Wang, Y., Jiang, H., Yu, D., Hu, M. (2014). Effect of Silica-Based Nanomaterials and Their Derivate with PEGylation on Cementoblasts. Annals of Biomedical Engineering, 42 (8): 1781 ? 1789. PMid:24752634View Article PubMed/NCBI
Kell, A. J., Barnes, M. L., Slater, K., Lavigne, C. (2012). Functionalised silica nanoparticles stable in serum-containing medium efficiently deliver siRNA targeting HIV-1 co-receptor CXCR4 in mammalian cells. International Journal of Nano and Biomaterials 2, 4(3-4): 223 ? 242.
Liu, X., Qian, H., Ji, Y., Li, Z., Shao, Y., Hu, Y., Tong, G. X., Li, L., Guo, W., Guo, H. (2012). Mesoporous silica-coated NaYF4 nanocrystals: facile synthesis, in vitro bioimaging and photodynamic therapy of cancer cells. RSC Advances, 2(32): 12263 ? 12268.View Article
Natarajan, S. K., Selvaraj, S. (2014). Mesoporous silica nanoparticles: Importance of surface modifications and its role in drug delivery. RSC Advances, 4: 14328 ? 14334.View Article
Monnier, A., Schu?th, F., Huo, Q., Kumar, D., Margolese, D., Maxwell, R. S., Stucky, G. D., Krishnamurty, M., Petroff, P., Firouzi, A., Janicke, M., Chmelka, B. F. (1993). Cooperative formation of inorganic-organic interfaces in the synthesis of silicate mesostructures. Science, 261: 1299 ? 1303. PMid:17731857View Article PubMed/NCBI
Firouzi, A., Kumar, D., Bull, L. M., Besier, T., Sieger, P., Huo, Q., Walker, S. A., Zasadzinski, J. A., Glinka, C., Nicol, J., Margolese, D., Stucky, G. D., Chmelka, B. F. (1995). Cooperative organization of inorganic-surfactant and biomimetic assemblies. Science, 267: 1138 ? 1143. PMid:7855591View Article PubMed/NCBI
Huo, Q., Margolese, D. I., Ciesla, U., Demuth, D. G., Feng, P., Gier, T. E., Sieger, P., Firouzi, A., Chmelka, B. F.; Schu? th, F. Stucky, G. D. (1994). Organization of Organic Molecules with Inorganic Molecular Species into Nanocomposite Biphase Arrays. Chemistry of Materials, 6: 1176 ? 1191.View Article
Anderson, M. T., Martin, J. E., Odinek, J. G., Newcomer, P. P. (1998). Surfactant-Templated Silica Mesophases Formed in Water: Cosolvent Mixture. Chemistry of Materials, 10: 311.View Article
Gru?n, M., Unger, K. K., Matsumoto A., Tsutsumi, K. (1999). Novel pathways for the preparation of mesoporous MCM-41 materials: control of porosity and morphology. Microporous and Mesoporous Materials, 27: 207 ? 216. 00255-8View Article
Biswas, M., Majumdar, S., Chowdhury, T., Chattopadhyay, B.D., Mandal, S., Halder, U., Yamasaki, S. (2010). Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology. Enzyme and Microbial Technology, 46: 581 ? 587.View Article
Chowdhury, T., Sarkar, M., Chaudhuri, B., Chattopadhyay, B.D., Halder, U.C. (2015). Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein. Journal of Biological Inorganic Chemistry, 20(5): 791 ? 803. PMid:25944503View Article PubMed/NCBI
Show, S., Tamang, A., Chowdhury, T., Mandal, D., Chattopadhyay, B.D. (2015). Bacterial (BKH1) Assisted Silica Nanoparticles from Silica Rich Substrates: A Facile and Green approach for biotechnological applications. Colloids and Surfaces B: Biointerfaces, 126: 245 ? 250. PMid:25576815View Article PubMed/NCBI
Show, S., Chattopadhyay, B.D. (2016). Protein Mediated Silica Particles with pH Controlled Porosity and Morphology. Advances in Microbiology, 6: 986 ? 998.View Article
Wei, Z., Yan, P., Feng, W., Dai, J., Wang, Q., Xia, T. (2006). Microstructural characterization of Ni nanoparticles prepared by anodic arc plasma. Materials Characterization, 57(3): 176 ? 181.View Article
Unger, K.K. (1979). Porous Silica. Journal of Chromatography Library, 16: 237-242.
Umari, P., Pasquarello, A. (2003). First-principles analysis of the Raman spectrum of vitreous silica: comparison with the vibrational density of states, Journal of Physics: Condensed Matter, 15(16): S1547 ? S1552.View Article
Anedda, A., Carbonaro, C.M., Clemente, F., Corpino, R., Ricci, P. C. (2003). Raman Investigation of Surface OH-Species in Porous Silica. Journal of Physical Chemistry B, 107 (49): 13661 ? 13664.View Article
Anedda, A., Carbonaro, C.M., Clemente, F., Corda, L., Corpino, R., Ricci, P.C. (2003). Surface hydroxyls in porous silica: a Raman spectroscopy study, Materials Science and Engineering: C, 23 (6-8): 1069 ? 1072.View Article
Galeener, F.L. (1982). Planar rings in vitreous silica, Journal of Non-Crystalline Solids, 49(1-3): 53 ? 62. 90108-9View Article
Alessi, A., Agnello, S., Buscarino, G., Gelardi, F.M. (2013). Structural properties of core and surface of silica nanoparticles investigated by Raman spectroscopy. Journal of Raman Spectroscopy, 44(6): 810 ? 816.View Article