A highly-sensitive and selective antibody-like sensor based on molecularly imprinted poly(L-arginine) on COOH-MWCNTs for electrochemical recognition and detection of deoxynivalenol.

Affiliation

Li W(1), Diao K(2), Qiu D(1), Zeng Y(1), Tang K(3), Zhu Y(4), Sheng Y(4), Wen Y(5), Li M(4).
Author information:
(1)College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China; Institute of Functional Materials and Agricultural Applied Chemistry, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
(2)College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, People's Republic of China.
(3)College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China. Electronic address: [Email]
(4)Institute of Functional Materials and Agricultural Applied Chemistry, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
(5)Institute of Functional Materials and Agricultural Applied Chemistry, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China. Electronic address: [Email]

Abstract

A new strategy to mimic antibody for electrochemical recognition and detection of deoxynivalenol (DON) using a highly-sensitive and selective antibody-like sensor based on molecularly imprinted poly(l-arginine) (P-Arg-MIP) on carboxylic acid functionalized carbon nanotubes (COOH-MWCNTs) was proposed. l-arginine as functional monomer was screened to prepare imprinted electrode via its electro-polymerization in the presence of DON onto the surface of COOH-MWCNTs electrode coupled with theoretical calculation. Surface morphology, structural characteristics, and electrochemical properties of P-Arg-MIP/COOH-MWCNTs were characterized by SEM, EDS, FTIR, and CV, respectively. P-Arg-MIP/COOH-MWCNTs displayed relatively high conductivity, high effective surface area, antibody-like molecular recognition and affinity, and a good response towards DON in a linear range from 0.1 to 70 μM with LOD of 0.07 μM in wheat flour samples with satisfactory recovery and feasible practicability in comparison with HPLC. This method provides a promising biomimetic sensing platform for the determination of mycotoxins in food and agro-products.