Journal of Food Science & Technology

ISSN: 2472-6419

Impact Factor: 1.343

VOLUME: 8 ISSUE: 1

Page No: 479-491

Repairing and Protecting Effects of Pneumatophorus japonicus heads peptides on GES-1 Cells Damaged by Alcohol


Corresponding Author

C.H. Xu,

College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.

E-mail addresses: hongmiaosi@126.com (M. -S. Hong), chxu@shou.edu.cn (C.-H. Xu)

Co-Authors

Yi-Ying Tanga,e,1, Ming-Hui Gaoa,e,1, Jie-Shuai Zhanga, Ning-ping Taoa, b, c, d, Xi-Chang Wanga, b, c, d, Ping Wangf, Shang-Gui Dengg, Miao-Si Hongh,*, Ying Lua, Jing-Ya Yanga, Chang-Hua Xua, b, c, d*

Citation

Yi-Ying Tang, Ming-Hui Gao, Jie-Shuai Zhang, Ning-ping Tao, Xi-Chang Wang, Ping Wang, Shang-Gui Deng, Miao-Si Hong, Ying Lu, Jing-Ya Yang, Chang-Hua Xu, Repairing and Protecting Effects of Pneumatophorus japonicus heads pep- tides on GES-1 Cells Damaged by Alcohol(2023) Journal of Food Science & Technology 8(1) : 479-491

Abstract

Stomach mucosal damage posed a great impact on stomach health and may escalated to stomach cancer. Given that gastric mucosal epithelial cells (GES-1) were the foremost essential part of gastric mucosal defense barrier, their repair and protec- tion were crucial for gastric strengthening. In this study, based on "macro-micro-nano-level" molecular spectroscopy, a multi -scale in-situ analysis approach of microscopic imaging, complemented by cellular biological methods, was proposed to systematically investigate the dynamic regulation of home-developed Pneumatophorus japonicus head peptides (PHPs) on the visual-chemical structure and composition distribution of alcohol-damaged GES-1, and subsequently to reveal the inter- action mechanism between PHPs and GES-1 components. The results showed that 3-10 kDa PHPs had superior repair and protection effects compared to gastric mucin, significantly promoted cell proliferation, reduced the leakage of lactate dehy- drogenase (LDH), and increased the mitochondrial membrane potential (MMP). It was deduced that PHPs promoted synthe- sis of glycoproteins, enhanced superoxide dismutase (SOD) and inhibited production of reactive oxygen species (ROS), thus achieving protection and repair functions on GES-1. This study provided theoretical and technical reference for the develop- ment of new natural gastric repair peptide products with high activity in future.

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