A novel electrochemical enzyme biosensor for detection of 17β-estradiol by mediated electron-transfer system.

Affiliation

Department of Chemistry, Shanghai University, Shanghai 200444, PR China; Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University, Shanghai 200444, PR China. Electronic address: [Email]

Abstract

An extremely sensitive enzyme sensor for detection of 17β-estradiol based on electropolymerized L-lysine molecules on a glassy carbon electrode (GCE) modified with critic acid@graphene (CA-GR) and cross-linked with laccase enzyme has been developed in this work. As the laccase immobilization, glutaraldehyde was chosen as cross-linker through the groups reactions. The novel enzyme sensor could recognize and determinate 17β-estradiol effectively. The morphology of the enzyme modified electrode was characterized by transmission electron microscopy (TEM) and electron microscopy (SEM). The amino interaction between cross-linker and enzyme was characterized by Fourier transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, good linear relationships were achieved in the range of 4 × 10-13 - 5.7 × 10-11 M and a limit of detection as low as 1.3 × 10-13 M. Moreover, the enzyme sensor exhibited good reproducibility, stability and high selectivity to 17β-estradiol. Excellent performance was showed in the human urine samples analysis, thus confirming great prospect for further application in clinic diagnosis and biological research.

Keywords

17β-estradiol,Covalent immobilization,Enzyme biosensor,Poly L-lysine,