A porous organic polymer with magnetic nanoparticles on a chip array for preconcentration of platinum(IV), gold(III) and bismuth(III) prior to their on-line quantitation by ICP-MS.


Department of Chemistry, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Wuhan University, Wuhan, 430072, China. [Email]


A chip-based array is described for magnetic solid-phase microextraction (MSPME) of the ions of Pt, Au and Bi. Magnetic porous organic polymers (MOPs) prepared from magnetite nanoparticles and 1,3,5-tris(4-aminophenyl)benzene are introduced as a novel adsorbent. Eight solid phase extraction columns packed with MOPs were integrated in parallel on a microfluidic chip for array microextraction. After elution with a 12% (m/v) solution of cysteamine hydrochloride (pH 8.0), the eluent is introduced into an ICP-MS instrument for quantification. Under the optimized conditions, the limits of detection for Pt, Au and Bi are 8.6, 4.4 and 3.4 ng L-1, respectively. The sample throughput is 7 h-1, and the adsorption capacities are 32, 24 and 24 μg mg-1 for Pt, Au and Bi, respectively. The method was validated by the determination of Bi in a certified reference material (GSH-1A; human hair), and the values obtained coincided with the certified value. This method was also applied to the determination of Pt, Au and Bi in (spiked) urine and cell samples, and good recoveries (85.8-113%) were achieved. The method is highly sensitive and has a high throughput and a low sample/reagent consumption (with 500 HeLa cells consumed). Graphical abstract Schematic presentation of the magnetic packed column, microfluidic chip, and online chip-based MSPME-ICPMS system. Design sketch of the online system: microextraction unit (blue lines), microvalves (black lines), outlet channels (yellow lines), permanent magnets (red), urine and cell samples.


Array chip-based MSPME,Cell,Diazocoupling reaction,GSH-1A reference material,ICP-MS,Magnetic packed column,On-line analysis,Thiol functional group,Throughput,Urine,