A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria.


Key Laboratory for Rare Disease Research of Shandong Province, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, 18877, Jingshi Road, Jinan, 250012, Shandong, China. [Email]


Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differential adhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.



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