A surface spectroscopy study of a Pseudomonas fluorescens biofilm in the presence of an immobilized air bubble.


Département de Chimie, Faculté des sciences et de génie, Université Laval, Québec City, QC, Canada; CHU de Québec, centre de recherche, Université Laval, 10 rue de l'Espinay, Québec, QC, Canada; Centre de recherche sur les matériaux avancés (CERMA), Canada; Centre québécois sur les matériaux fonctionnels (CQMF), Canada. Electronic address: [Email]


A linear spectral mapping technique was applied to monitor the growth of biomolecular absorption bands at the bio-interface of a nascent Pseudomonas fluorescens biofilm during and after interaction with a surface-adhered air bubble. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectra were obtained in different locations in a microchannel with adequate spatial and temporal resolution to study the effect of a static bubble on the evolution of protein and lipid signals at the ATR crystal surface. The results reveal that the presence of a bubble during the lag phase modified levels of extracellular lipids and affected a surface restructuring process, many hours after the bubble's disappearance.


Attenuated total reflection Fourier-transform (ATR-FTIR) spectroscopy,Bacterial biofilms,Biomolecules,Dynamic restructuring,Gas liquid interface,Microfluidics,

OUR Recent Articles