An in vitro long-term study of cryopreserved umbilical cord blood-derived platelet-rich plasma containing growth factors-PDGF-BB, TGF-β, and VEGF.

Affiliation

Department of Plastic and Aesthetic Surgery (Head of Department: Prof. Dr. A. Takeda), School of Medicine, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan; Department of Plastic Surgery (Head of Department: Dr. K. Baba), Kitasato University Medical Center, 6-100 Arai, Kitamoto, Saitama 364-8501, Japan. Electronic address: [Email]

Abstract

OBJECTIVE : Umbilical cord blood-derived platelet-rich plasma (UCB-PRP) containing growth factors has attracted attention as a biomaterial useful for regenerative medicine. The osteoblastic differentiation of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) can be induced by UCB-PRP.
METHODS : Nine samples of UC and UCB were used to conduct an in vitro study that determined the contents of three growth factors (i.e., platelet-derived growth factor, transforming growth factor β-1, and vascular endothelial growth factor) and that examined, by staining with Alizarin red, their ability to induce the osteoblastic differentiation of UC-MSCs at the baseline, 3 months, and 3 years of cryopreservation.
RESULTS : The contents of growth factors in cryopreserved UCB-PRP were markedly elevated compared to those found in UCB at baseline. The samples of UCB that were added with cryopreserved UCB-PRP and those with bone morphogenetic protein-2 were stained granularly with Alizarin red, thus indicating the presence of calcium. The samples of UCB that were not added with UCB-PRP were not stained with Alizarin red. The above-mentioned contents and ability were maintained at 3 years of cryopreservation. Cryopreserved UCB-PRP possibly and advantageously induced the osteoblastic differentiation of UC-MSCs.
CONCLUSIONS : The potential clinical application of cryopreserved UCB-PRP to regenerative medicine was suggested.

Keywords

Cryopreservation,Growth factor,Mesenchymal stromal cells,Platelet-rich plasma,Regenerative medicine,Umbilical cord blood,

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