TiO2/Sb2S3/GQDs nanocomposite was prepared by solvothermal method to study its antibacterial properties. XRD, SEM, EDX, FT-IR and TEM were used to characterize prepared nanomaterials. To evaluate effect of graphene quantum dots (GQD) on the antibacterial activity of final products, antibacterial activity of TiO2, TiO2/GQDs, Sb2S3, Sb2S3/GQDs were compared with TiO2/Sb2S3/GQDs. TiO2/Sb2S3/GQDs shows the highest antibacterial activity against E. coli and Staphylococcus aureus. TiO2/Sb2S3/GQDs show the lowest measure the minimal inhibitory concentration against E. coli and Staphylococcus aureus. MIC for E. coli and Staphylococcus aureus are 0.03 and 0.1, respectively.