Assessment of NMDA receptor inhibition of phencyclidine analogues using a high-throughput drebrin immunocytochemical assay.

Affiliation

Department of Neurobiology and Behavior, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. Electronic address: [Email]

Abstract

In recent years, new psychoactive substances (NPS) have been widely distributed for abuse purposes. Effective measures to counter the spread of NPS are to promptly legislate them through the risk assessment. Phencyclidine analogues having inhibitory effects toward NMDA receptor (NMDAR) have recently emerged in Japan. Therefore, it is important to establish a high-throughput system for efficiently detecting NPS that can inhibit NMDAR activity.

Keywords

Dendritic spine,Drebrin,High-throughput analysis,Immunocytochemistry,NMDA receptor antagonist,Phencyclidine,Primary neuronal culture,Psychoactive substances,Synapse,