Association of physical activity and fitness with S-Klotho plasma levels in middle-aged sedentary adults: The FIT-AGEING study.

Affiliation

EFFECTS-262 Research Group, Department of Medical Physiology, Faculty of Medicine, University of Granada, Spain; PROmoting FITness and Health Through Physical Activity Research Group (PROFITH), Department of Physical Education and Sports, Faculty of Sport Sciences, University of Granada, Spain. Electronic address: [Email]

Abstract

OBJECTIVE : To determine the association between physical activity and physical fitness levels (i.e. cardiorespiratory fitness and muscular strength) and the shed form of the α-Klotho gene (S-Klotho plasma levels) in middle-aged sedentary adults.
UNASSIGNED : A total of 74 middle-aged sedentary adults (52.7% women; 53.7 ± 5.1 years old) were enrolled in the FIT-AGEING study. Physical activity and sedentary time were assessed with a wrist-worn accelerometer. Maximal oxygen uptake (VO2max) was determined by a maximum treadmill test using indirect calorimetry. Lower- and upper-body muscular strength were assessed by an isokinetic strength test and by the hand grip strength test, respectively. The S-Klotho plasma levels were measured in the EDTA plasma using a solid-phase sandwich enzyme-linked immunosorbent assay.
RESULTS : Based on the principal-component analysis, overall physical activity, moderate and vigorous physical activity levels, and sedentary time (as outcomes included in the 'sedentary time and physical activity' category) explained a total of 17.5% of the cumulative variance in S-Klotho plasma levels, while extension peak torque, hand grip strength, and maximal oxygen uptake (as outcomes included in the 'physical fitness' category) explained a total of 15.5% of the cumulative variance in S-Klotho plasma levels. Based on the loading of variables in these 2 categories, the percentage of the cumulative variance in S-Klotho plasma level explained was 28.9%s; this reached 33.0% of cumulative variance when sex was included in the model.
CONCLUSIONS : In summary, our results indicate that physical activity and physical fitness levels are associated with S-Klotho plasma levels in middle-aged sedentary adults. Therefore, the S-Klotho protein could be a key factor in the relationship between physical activity and physical fitness and health improvements during the ageing process.

Keywords

Ageing,Hand grip,Longevity,Peak torque,VO(2)max,