Boosting fermentable sugars by integrating magnesium oxide-treated corn stover and corn stover liquor without washing and detoxification.


Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, United States. Electronic address: [Email]


Integration of MgO-treated corn stover and corn stover liquor for enzymatic hydrolysis was studied to improve sugar yield and concentration as well as to simplify the bioconversion process. Results showed that under the same enzymatic hydrolysis conditions (2 mL enzyme/g biomass and 10% substrate loading), MgO-treated corn stover plus corn stover liquor had a similar glucose yield (70.4 vs. 68.8%) and concentration (40.4 vs. 41.5 g/L) but a higher xylose yield (56.6 vs. 25.3%) and concentration (16.7 vs. 10.6 g/L) compared with using MgO-treated corn stover only. Corn stover slurry from MgO pretreatment was near-neutral and free of 5-hydroxymethylfurfural and furfural, eliminating the need for washing and detoxification and lightening the burden for wastewater treatment. In addition, Tween 80 significantly reduced the irreversible binding of lignin to enzyme, increasing xylose and glucose yields by 14.7 and 6.2% and sugar concentration by 7.4 g/L, respectively.


Biomass slurry,Corn stover,Enzymatic hydrolysis,MgO pretreatment,Sugar yield,

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