CHROMagar™ orientation urine culture medium produces matrix-assisted laser desorption ionization-time-of-flight mass spectrometry spectra misidentified as Mycoplasma arginini and Mycoplasma alkalescens.

Author

Philippe R S Lagacé-Wiens

Affiliation

Shared Health, Diagnostic Services, Clinical Microbiology, Winnipeg, Manitoba, Canada; Department of Medical Microbiology and Infectious Diseases, College of Medicine, University of Manitoba. Winnipeg, Manitoba, Canada. Electronic address: [Email]

Abstract

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry is commonly used to identify bacteria and yeasts. Studies indicate that MALDI-TOF is relatively indifferent to the medium used for culture. We report on an investigation into high- and low-confidence MALDI-TOF misidentifications of Mycoplasma arginini and Mycoplasma alkalescens from urine specimens plated to CHROMagar™ Orientation medium that appear to be due to the intrinsic mass spectrum of the medium.

Keywords

Chromogenic,Culture,Identification,MALDI-TOF,Mass spectrometry,Mycoplasma,Urine,

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