CRISPR in Parasitology: Not Exactly Cut and Dried!

Affiliation

Biology of Host-Parasite Interactions Unit, Institut Pasteur, 75015 Paris, France; INSERM, Unit U1201, Paris, France; CNRS, ERL 9195, Paris, France. Electronic address: [Email]

Abstract

CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing for the dissection of molecular processes with unprecedented efficiency. Optimization and implementation of a new technology like CRISPR, especially in nonmodel organisms, requires communication and collaboration throughout the field. Recently, a 'CRISPR in Parasitology' symposium was held at the Institut Pasteur Paris, bringing together scientists studying Leishmania, Plasmodium, Trypanosoma, and Anopheles. Here we share technological advances and challenges in using CRISPR/Cas9 in the parasite and vector systems that were discussed. As CRISPR/Cas9 continues to be applied to diverse parasite systems, the community should now focus on improvement and standardization of the technique as well as expanding the CRISPR toolkit to include Cas9 alternatives/derivatives for more advanced applications like genome-wide functional screens.

Keywords

Anopheles,CRISPR,CRISPRi,Leishmania,Plasmodium,Trypanosoma,

OUR Recent Articles