Characterization of an endonuclease in rice stripe tenuivirus Pc1 in vitro.


College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, People's Republic of China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, People's Republic of China. Electronic address: [Email]


Rice stripe tenuivirus (RSV) initiates its mRNA transcription by using the cap-snatching mechanism during which an endonuclease activity is required for the cleavage of host mRNA. In this study, we aim to characterize the endonuclease in RSV. Sequence alignment revealed the presence of a cap-snatching endonuclease domain in RSV Pc1. Expression and in vitro enzymatic activity assay demonstrated that this domain indeed had a manganese-dependent endonuclease activity. The enzyme could efficiently degrade ssRNA with preference for unstructured ssRNA, but not DNA. Mutations in the endonuclease domain allowed the identification of four key residues (D547, D567, E585 and K604). The endonuclease of RSV was similar but not identical to other known viral endonucleases, suggesting that RSV endonuclease may have some distinct catalytic characteristics.


Endonuclease activity,Key residues,Pc1,Rice stripe tenuivirus,