Characterization of neurite dystrophy after trauma by high speed structured illumination microscopy and lattice light sheet microscopy.


Department of Neurosurgery, NorthShore University HealthSystem, Evanston, IL, USA. Electronic address: [Email]


Unbiased screening studies have repeatedly identified actin-related proteins as one of the families of proteins most influenced by neurotrauma. Nevertheless, the status quo model of cytoskeletal reorganization after neurotrauma excludes actin and incorporates only changes in microtubules and intermediate filaments. Actin is excluded in part because it is difficult to image with conventional techniques. However, recent innovations in fluorescent microscopy provide an opportunity to image the actin cytoskeleton at super-resolution resolution in living cells. This study applied these innovations to an in vitro model of neurotrauma.


Dystrophy,Human induced pluripotent stem cell derived neurons,Lattice light sheet microscopy,Structured illumination microscopy,Traumatic brain injury,