Chitosan capped ZnO nanoparticles with cell specific apoptosis induction through P53 activation and G2/M arrest in breast cancer cells - In vitro approaches.

Affiliation

Division of Entomology, Department of Zoology, Bharathiar University, School of Life Science, Coimbatore 641 046, Tamil Nadu, India. Electronic address: [Email]

Abstract

Now a days the well-organized strategy to induce apoptosis in cancer chemotherapy is to produce anti-cancer agent without any side effects is in needy. Hence the present investigation was aimed to explore the anticancer potentials of Amorphophallus paeoniifolius reduced zinc nanoparticles capped with chitosan against MCF 7 cell line (breast cancer cells)and studied for its optical and surface charge properties. The size, shape, dispersion and uniform distribution of biosynthesized zincoxide nanoparticle was examined using Field emission scanning electron microscope (FESEM) and Transmission electron microscope (TEM) respectively. The spherical and cubic nanocrystals were found to be lethal against MCF 7 cells on MTT assay at dose dependant manner (20-80 μg/ml) whose IC50 value 42 μg/ml. Bright field light microscopic study showed the apoptotic morphology of treated and control MCF-7 cells. Fluorescence staining A/O:EB and DAPI methods further cleared the chromosome condensation, nuclear fragmentation and confirms the apoptosis induced by Ch-Ap-ZnONPS within IC50 concentrations. Significant cell cycle arrest at particular stage of G2/M was achieved with the nanocomplex treatment at dose dependant manner. Finally, it was observed that the apoptotic gens and protein expressions of MCF-7 cell line were up and down regulation with the treatment of Ch-Ap-ZnONPS when compared to normal cells.