Health of transition goats can be explored through the assessment of the metabolic profile. Selected circulating parameters of clinical importance may be used for indicating homeostasis perturbations during the transition period. The present study aimed to characterize the metabolic profile of transition dairy goats raised under a pasture-based system to determine whether the conceptus number (single vs. twin gestation) influences circulating concentrations of different parameters in the bloodstream, including nutrient- and fluid-related metabolites for health assessment. Sarda dairy goats diagnosed for single (n = 5) or twin gestation (n = 6) were selected out of a flock of 156 heads and sampled for blood weekly, throughout one month before and one month after kidding. Significantly (p < .01), differences in blood serum metabolite concentrations before and after birthing were observed in both groups of animals, as to glucose (Glu), total cholesterol (TC) and triglycerides (TG), total protein (TP) and Urea. Circulating creatinine (Crea) turned out to differ significantly (p < .01) between single vs. twin kidding does. Significant correlations (p < .001) between groups of circulating electrolytes (Na, Chloride, Mg, K) were also observed and this datum was assumed to change following the different distribution of fluids in the body of the doe, in the last third of gestation, at birthing and during early lactation, respectively. Interestingly, electrolyte levels in this trial displayed different concentrations in the bloodstream according to physiological stage and kid numbers.