The purposes of this study are to conduct an in vitro comparison of the shear bond strength, breakage mode, and wire deformation of three different types of retainers and to compare the subsequent enamel surface changes. Two hundred seventy intact lower incisor teeth were embedded in acrylic blocks in pairs. Dead wire and CAD/CAM-fabricated and fiber-reinforced wires were applied to the teeth roughened with acid and Er:YAG or Er,Cr:YSGG laser. The surface roughness was observed by scanning electron and atomic force microscopy. The samples were analyzed for shear bonds. The dead wire and acid group were found to have the highest bonding strength and the strengths for all groups in which acid was used as an agent were found to be higher than others. Deformation of retainers was most noted in the dead wire-acid group. Among all the groups, the CAD/CAM-fabricated wire group showed the least deformation, with no deformation observed. In this study, it was determined that there is a significant correlation between ARI scores and agents. Consequently, acid etching was found to create more enamel surface roughness than laser groups. It was also seen that the combined use of the acid method and dead soft wire had the highest bond strength, even though it was not statistically significant. It was concluded that CAD/CAM-fabricated wire provides the opportunity for reuse in clinical applications due to its lack of deformation, being more conservative for the patient, and being more advantageous for the clinician in terms of session time, considering the residual adhesive amount left on the enamel surface.