Development of a multiplex real-time PCR assay for rapid detection of tigecycline resistance gene tet(X) variants from bacteria, faeces, and environment samples.


Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China [Email] [Email]


We developed a multiplex real-time SYBR Green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1), tet(X2), and high-level tigecycline resistance genes tet(X3), tet(X4) and tet(X5). High linearity (R2 ≥0.996), sensitivity (low detection limit) and specificity (only the target gene could be amplified significantly) were showed in developed real-time PCR assay and further evaluated using bacterial, faeces, and environmental samples.

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