Effect of the desiccation tolerance and cryopreservation methods on the viability of Citrus limon L. Burm cv. Eureka seeds.

Affiliation

Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CONICET, Facultad de Ciencias Exactas UNLP, CIC-PBA, Argentina), Calle 47 y 116 La Plata, Buenos Aires, Argentina. Electronic address: [Email]

Abstract

Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influence on the interval of optimal moisture content in the desiccation stage were analyzed. Water sorption isotherm was determined and modeled using D'Arcy & Watt equation; it provided important information concerning the amounts of water associated to strong, weak and multimolecular binding sites along the sorption isotherm. Seeds tolerated a wide range of desiccation conditions (0.180%), however desiccation to 0.0526 g H2O g-1 d.b. (aw = 0.0901) produced a significant loss of viability. Differential Scanning Calorimetry was used to identify the thermal transitions of lipids and water in the seed; enthalpies were used to calculate the unfrozen water fraction (0.19 g H2O g-1 d.b. corresponding to aw = 0.64). Two cooling/warming rates were tested on desiccated seeds (0.110.64 corresponding to the unfrozen water fraction. The use of higher cooling/warming rates enables a wider range of desiccation conditions (0.33

Keywords

Cooling/warming rates,Cryopreservation,Desiccation tolerance,Sorption isotherm,Thermal transitions,Viability,Water activity,

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