Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor.


Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, Saltillo, Coahuila 25280, Mexico; Cluster of Bioalcohols, Mexican Centre for Innovation in Bioenergy (Cemie-Bio), Mexico. Electronic address: [Email]


One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 °C/30 min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.


Autohydrolysis,Biofuels,Biorefinery,Glucan,Glucose,Severity factor,

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