Epitope approach in molecular imprinting of antibodies.


Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Chile. Electronic address: [Email]


Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMIPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMIPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation.


Affinity chromatography,Antibodies,Molecular imprinting polymers,Nanoparticles,Protein epitope,Solid-phase synthesis,

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