Fabrication of fish gelatin / sodium alginate double network gels for encapsulation of probiotics.

Affiliation

Liu J(#)(1)(2), Liu F(#)(1)(3), Ren T(#)(1)(4), Wang J(1)(5), Yang M(1), Yao Y(1), Chen H(1)(6).
Author information:
(1)Marine College, Shandong University, Weihai, China.
(2)State Key Lab Food Science and Technology, Jiangnan University, Wuxi, China.
(3)CAS Key Laboratory of Experimental Marine Biology, CAS Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
(4)Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
(5)College of Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
(6)Beijing Advanced Innovation Centre for Food Nutrition and Human Health, Beijing Technology and Business University
(BTBU), Beijing, China.
(#)Contributed equally

Abstract

BACKGROUND: To improve the environmental resistance of probiotics, and particularly their survival in the gastrointestinal environment, a fish gelatin (FG) / sodium alginate (SA) double network gelation (FSDN) was developed to encapsulate them. Thermal treatment and calcium ion inducement were adopted to fabricate fish gelatin and sodium alginate gels. It was feasible to scale up this process. The effects of FG concentration (0-60 g/L) on FSDN properties, including morphology, water-holding capacity, and encapsulation efficiency were evaluated. RESULTS: The results indicated that the addition of FG could improve the transparency, rehydration, and water-holding capacity of FSDN. Scanning electronic microscope (SEM) images revealed that FSDN had a denser and more complete structure than SA. Encapsulation efficiency improved from 15.85% to 91.91% as the FG concentration ranged from 0 to 50 g/L. Bifidobacterium longum embedded by FSDN showed better thermal stability than when it was free. Compared with bare probiotics (1.7%), the encapsulated ones exhibited higher viability (above 15%) in simulated gastric fluid. CONCLUSION: In conclusion, interpenetrating FSDN is an effective barrier constituent and could achieve the targeted delivery of probiotics. It is a potential new delivery carrier for the oral administration of probiotics. © 2021 Society of Chemical Industry.