Feasibility of trifluoromethyl TROSY NMR at high magnetic fields.


Department of Biochemistry, University of Alberta, Edmonton, AB, T6G2H7, Canada. [Email]


Insights into the structure and dynamics of large biological systems has been greatly improved by two concurrent NMR approaches: the application of transverse relaxation-optimized spectroscopy (TROSY) techniques in multi-dimensional NMR, especially the methyl-TROSY, and the resurgence of 19F NMR using trifluoromethyl (CF3) probes. Herein we investigate the feasibility of combining these approaches into a trifluoromethyl-TROSY experiment. Using a CF3-labelled parvalbumin, we have evaluated the natural abundance 13C-19F correlation spectra and find no indication of a CF3 TROSY at high magnetic fields.


19F NMR,Chemical shift anisotropy,High magnetic fields,Methyl-TROSY,Trifluoromethyl,