Lee D(1), Lee JW(2), Kim C(1), Lee D(2), Chung S(3), Yoon DS(4), Lee JH(5). Author information:
(1)Department of Electrical Engineering, Kwangwoon University, Seoul, 01897,
Republic of Korea.
(2)CALTH. Inc. Changeop-ro 54, Seongnam, Gyeonggi, 13449, Republic of Korea.
(3)School of Mechanical Engineering, Korea University, Seoul, 02841, Republic of
(4)School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of
Korea. Electronic address: [Email]
(5)Department of Electrical Engineering, Kwangwoon University, Seoul, 01897,
Republic of Korea. Electronic address: [Email]
Micro/nanofluidics are excellent candidates for biological sample preparation. However, the limited process volume in micro/nanofluidics is the main hurdle limiting their practical applications. To date, most micro/nanofluidics have processed sample volumes of several microliters and have rarely been used to handle large-volume samples. Herein, we propose a microfluidic paper-based large-volume preconcentrator (u-LVP) for enrichment and purification of biomarkers (e.g., miRNA) using ion concentration polarization. A Nafion (ion-selective nanoporous membrane)-functionalized multilayer cellulose paper enables microscale division of milliliter-scale samples, thus electrokinetically separating and preconcentrating the biomarker in different locations within the u-LVP. By inserting collecting discs at optimal positions in the u-LVP, the enriched biomarker is simply recovered with high efficiency. With this approach, as an exemplary biomarker, miRNA-21 in human serum was separated from proteins and preconcentrated with an effective preconcentration factor exceeding 6.63 and a recovery rate above 84%. Thus, our platform offers new opportunities and benefits for biomarker, diagnostic, prognostic, and therapeutic research.
Having over 250 Research scholars worldwide and more than 400 articles online with open access.