Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina.

Affiliation

Department of Biology, University of York, York, North Yorkshire, YO10 5DD, UK. [Email]

Abstract

Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify more integrating vectors, using phage int/attP loci, that would efficiently integrate site-specifically in the rare Actinomycete, Amycolatopsis marina DSM45569.

Keywords

Amycolatopsis,Integrating vectors,R4 integrase,Rare Actinomycetes,TG1 integrase,

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