The aim of this study was to investigate the effect of sprint interval exercise (SIT) and psychological need-support in exercise on post-exercise appetite and energy intake. Forty physically inactive men and women (BMI 24.6 ± 4.8 kg·m-2, V̇O2peak 26.6 ± 4.9 mL·kg-1·min-1) were randomised to either a need-support or no-support condition, with each participant completing two experimental trials involving 30 min of moderate-intensity continuous cycling (MICT; 60% V̇O2peak) and SIT (alternating 15 s at 170% V̇O2peak and 60 s at 32% V̇O2peak) matched for total work. Perceptions of appetite and appetite-related blood variables were assessed, together with ad libitum energy intake for three hours following exercise using a laboratory test meal and available snacks. Greater enjoyment, perceived exertion, heart rate, and blood lactate were observed in SIT compared with MICT (all p ≤ 0.006). Ratings of perceived appetite were similar across conditions and trials (p > 0.05); however, active ghrelin was lower following SIT compared with MICT (p < 0.001), and there was a significant condition-by-type interaction for energy intake (p = 0.033), with participants in the support group consuming less energy from foods following SIT (1895 ± 1040 kJ) than MICT (2475 ± 1192 kJ). Findings from this work highlight the need to reconsider traditional exercise guidelines where dietary intake is a concern. Novel points: - Enjoyment was greater during SIT compared with MICT - Enjoyment and choice were higher among participants provided with psychological need-support - In a need-supportive environment, SIT reduced subsequent energy intake compared with MICT.