LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203.

Affiliation

Department of Orthopedics, Jining No. 1 People's Hospital, Jining 272011, China. Electronic address: [Email]

Abstract

OBJECTIVE : PMS1 Homolog 2, Mismatch Repair System Component Pseudogene 2 (PMS2L2) has been reported as an up-regulated long non-coding RNA (lncRNA) in osteoarthritis (OA) tissues. The purpose of the present work is to explore whether the differently expressed PMS2L2 is associated with the pathogenesis of OA.
METHODS : Chondrogenic ATDC5 cells were exposed to various doses of lipopolysaccharide (LPS). The expression of PMS2L2, miR-203, and MCL-1 in cell was altered by transfection. Thereafter, cell viability, apoptosis, the expression changes of apoptosis-related factors and the release of pro-inflammatory factors were respectively assessed. Moreover, the regulatory relationship between PMS2L2 and miR-203, as well as between miR-203 and MCL-1 were studied.
RESULTS : PMS2L2 expression was down-regulated following LPS stimulation. PMS2L2 protected ATDC5 cells against LPS-induced injury by increasing cell viability, decreasing apoptosis, and repressing the release of pro-inflammatory factors. Meanwhile, PMS2L2 increased the expression levels of COL2A1 and ACAN, while down-regulated the expression levels of MMP13 and ADAMTS-5. PMS2L2 worked as a molecular sponge for miR-203. Besides, miR-203 overexpression partially abolished the chondroprotective effects of PMS2L2. MCL-1 was a direct target of miR-203, and it exerted the similarly chondroprotective effects as PMS2L2. Furthermore, PMS2L2 and MCL-1 blocked Wnt/β-Catenin and JAK/STAT signaling pathways also via a miR-203-dependent manner.
CONCLUSIONS : Our study reveals a protective role of PMS2L2 in LPS-induced inflammatory injury in chondrocytes. PMS2L2/miR-203/MCL-1 axis may serve as a new gene therapy strategy for the treatment of OA.

Keywords

Lipopolysaccharide (LPS),MCL-1,Osteoarthritis,PMS2L2,lncRNA,miR-203,