Sun B(1), Hayashi M(2), Kudo M(2), Wu L(3), Qin L(4), Gao M(2)(5), Liu T(3). Author information:
(1)Second Clinical Medical College, Beijing University of Chinese Medicine,
(2)School of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
(3)Key Laboratory of Health Cultivation of the Ministry of Education, Beijing
University of Chinese Medicine, Beijing, China.
(4)Technology Department, Beijing University of Chinese Medicine, Beijing,
(5)Institute for Biosciences, Mukogawa Women's University, Hyogo, Japan.
BACKGROUND: Pre-clinical research studies have shown that Madecassoside (MA) has favorable therapeutic effects on arthritis, acne, vitiligo and other diseases. However, the effects of MA on obesity have not yet been studied. This study mainly aimed to investigate the effects of MA in protecting against obesity and its underlying mechanism in reducing obesity. METHODS: Obese diabetic KKay/TaJcl mice model was adopted to the study. The body weight of all animals was recorded daily, and the blood glucose, blood lipid, and serum aminotransferase levels were examined, respectively. The expression of P-AMPK, SIRT1, P-LKB1, P-ACC, and P-HSL in abdominal fat, mesenteric fat, and epididymal fat was measured by western blotting, and the levels of PPARα, CPT1a, PGC-1α, UCP-1, Cidea, Cox7a1, and Cox8b were examined by real-time quantitative PCR (RT-qPCR). RESULTS: The results revealed that the body weight of the mice in MA group was significantly reduced, and the body mass index (BMI) showed significant difference between the two groups after 8 weeks of MA treatment. Further research revealed that it affected the mesenteric fat and epididymis fat by activating SIRT1/AMPK signaling pathway, and then promoted fatty acid oxidation of epididymal fat (PPARα ↑, CPT1a↑, and PGC-1α↑). Last but not the least, it also promoted the expression of UCP-1 and stimulated thermoregulatory genes (Cidea, Cox7a1, and Cox8b) in brown fat and mesenteric fat. CONCLUSIONS: Taken together, these findings suggest that MA can inhibit the weight gain in obese diabetic mice, and reduce triglyceride levels, inhibit lipogenesis of mesenteric fat, promote epididymal fat lipolysis and fatty acid oxidation. Furthermore, MA treatment might promote mesenteric fat browning and activate mitochondrial function in brown fat as well as mesenteric fat.
Having over 250 Research scholars worldwide and more than 400 articles online with open access.