Soil contamination with diesel oil is frequent and methods to improve remediation of diesel oil contaminated soils are urgently needed. The aim of the current study was to assess the potential of meat and bone meal (MBM) as a biostimulation agent to enhance diesel oil degradation in contaminated soils collected from southern Finland. MBM (2% w/w) increased oil degradation in soils when compared to natural attenuation. The increase was comparable to soils treated with a traditional fertilizer (urea). Soil pH increased rapidly in urea treated soil but remained at the level of natural attenuation in MBM treated soil, suggesting that in large-scale experiments MBM treated soils avoid the usual negative impact of urea on soil pH and ultimately microbial degradation. These results indicate that MBM addition enhances diesel oil degradation, and that MBM speeds up ex situ bioremediation of oil contaminated soils.