Wang R(1)(2), Lenka SK(1), Kumar V(1), Gashu K(3), Sikron-Persi N(3), Dynkin I(4), Weiss D(2), Perl A(4), Fait A(3), Oren-Shamir M(1). Author information:
(1)Department of Ornamental Plants and Agricultural Biotechnology, Agricultural
Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159,
Rishon LeZion 7505101, Israel.
(2)Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith
Faculty of Agriculture, Food, and Environment, The Hebrew University of
Jerusalem, Rehovot 76100, Israel.
(3)Ben-Gurion University of the Negev, Jacob Blaustein Insts. for Desert
Research, French Associates Institute for Agriculture & Biotechnology of
Drylands, Midreshet Ben-Gurion 849900, Israel.
(4)Department of Fruit Tree Sciences, Agricultural Research Organization, The
Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, Israel.
Stilbenes are phytoalexins with health-promoting benefits for humans. Here, we boost stilbenes' production, and in particular the resveratrol dehydrodimer viniferin, with significant pharmacological properties, by overexpressing stilbene synthase (STS) under unlimited phenylalanine (Phe) supply. Vitis vinifera cell cultures were co-transformed with a feedback-insensitive E. coli DAHP synthase (AroG*) and STS genes, under constitutive promoters. All transgenic lines had increased levels of Phe and stilbenes (74-fold higher viniferin reaching 0.74 mg/g DW). External Phe feeding of AroG* + STS lines caused a synergistic effect on resveratrol and viniferin accumulation, achieving a 26-fold (1.33 mg/g DW) increase in resveratrol and a 620-fold increase (6.2 mg/g DW) in viniferin, which to date is the highest viniferin accumulation reported in plant cultures. We suggest that this strategy of combining higher Phe availability and STS expression generates grape cell cultures as potential factories for sustainable production of stilbenes with a minor effect on the levels of flavonoids.
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