Carboxylated carbon porous adsorbent was derived from zeolite imidazole framework (ZIF-8) via carbonization of ZIF-8 under a nitrogen atmosphere. The synthesized carboxylated adsorbent was fully characterized by various techniques including Fourier transform spectroscopy (FTIR), powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and zeta potential analysis. The carboxylated adsorbent was applied as dispersive solid phase extraction (DSPE) adsorbent for efficient extraction of methamphetamine (MET) from biological urine samples. Several extraction parameters influencing the extraction efficiency were investigated and the calibration curve was plotted under optimized conditions in urine media. The method showed a good linearity in the range of 50-2500 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) was 10 and 35.80 ng/mL, respectively. A satisfactory analysis of the positive real samples with the recovery of 99.83% confirms the applicability of the proposed method in different clinical and forensic laboratories.