Novel influenza inhibitors designed to target PB1 interactions with host importin RanBP5.

Affiliation

Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602, USA. Electronic address: [Email]

Abstract

In search of novel targets for influenza inhibitors, a site on PB1 was selected for its high conservation and probable interaction with a host protein, RanBP5, that is key to nuclear import of PB1, where it complexes with PB2, PA, and NP to transcribe viral RNA. Docking with libraries of drug-like compounds led to a selection of five candidates that bound tightly and with a pose likely to inhibit protein binding. These were purchased and tested in vitro, found to be active, and then one was synthetically expanded to explore the structure-activity relationship. The top candidates had a carboxylic acid converted to an ester and electron-withdrawing substituents added to a phenyl group in the original structure. Resistance was slow to develop, but cytotoxicity was moderately high. Nuclear localization of PB1 and in vitro polymerase activity were both strongly inhibited.

Keywords

Antiviral,Nuclear localization,Polymerase inhibition,Virtual screening,

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