Polypyrrole-Modified Nylon 6 Nanofibers as Adsorbent for the Extraction of Two β-Lactam Antibiotics in Water Followed by Determination with Capillary Electrophoresis.


School of Public Health, and Key Laboratory of Environment and Human Health of Hebei Province, Hebei Medical University, Shijiazhuang 050017, China. [Email]


A solid phase membrane adsorbent-a nylon 6 nanofibers membrane coated by polypyrrole (PPy-PA6-NFsM)-was firstly synthesized and used for extraction of two β-lactam antibiotics (oxacillin and cloxacillin) in urban river water. Then the analytes were detected by capillary electrophoresis with a diode array detector (CE-DAD). The synthesized nanofibers membrane was characterized by scanning electron microscopy and a Fourier transform infrared spectrometer. The experimental conditions were optimized, including the amount used of PPy-PA6-NFsM, pH of the sample solutions, adsorption volume, and desorption conditions. Under the optimal extraction and separation conditions, the detection limits were found to be 2.0 ng/mL for both oxacillin and cloxacillin. The proposed method was applied to the determination of the two β-lactams in water samples of an urban river. The recoveries of these two β-lactams were found to be in the range 84.2-96.4%, demonstrating that PPy-PA6-NFsM has a high extraction capability for these two antibiotics. The relative standard deviations, ranging from 2.26% to 5.29% for intraday measurements and from 2.38% to 7.02% for inter-day determinations, were derived respectively.


CE-DAD,PPy-PA6-NFsM,determination,solid phase extraction,β-lactam residues,