Potential in vivo transfer of a blaCTX-M14-harbouring plasmid established by combining long- and short-read sequencing.

Affiliation

Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Antwerp, Belgium. Electronic address: [Email]

Abstract

Horizontal transfer of plasmid-mediated antibiotic resistance has rarely been documented in vivo. Utilizing long-read (Oxford Nanopore) and short-read (Illumina) sequencing, we confirmed that a gut-colonizing Escherichia coli and a hypervirulent Klebsiella pneumoniae ST23, isolated from a surgical site culture of a patient receiving cefuroxime therapy, harboured a 100% identical blaCTX-M-14-harbouring plasmid, indicative of a potential transfer in vivo.

Keywords

E coli,ESBL,Extended-spectrum beta-lactamases,Horizontal transfer,IncFII plasmid,K. pneumoniae,Nanopore sequencing,ST23,

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