Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China; State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, 266071, China. Electronic address: [Email]
In recent decades, ionic liquid-modified packing materials as stationary phases of high performance liquid chromatography (HPLC) have attracted widespread attention due to their comprehensive properties. In this work, monodisperse porous polymeric ionic liquid microspheres were directly prepared using a seeded emulsion polymerization method and applied as stationary phase of HPLC. The effects of porogen, monomer and crosslinker on the properties of porous P(IL-DVB) microspheres were investigated in detail. Porous P(IL-DVB) microspheres with good stability, monodispersity and relatively high specific surface area were selected as stationary phase to successfully separate benzene and its homologues, and nucleosides and alkaloids under reversed phase chromatography (RPLC) mode and hydrophilic interaction chromatography (HILIC) mode, respectively.