Protective effects of l-arginine against testosterone synthesis decreased by T-2 toxin in mouse Leydig cells.

Affiliation

College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, 471023, China; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, 471023, China. Electronic address: [Email]

Abstract

The testosterone levels decreased by T-2 toxin in mouse Leydig cells were reported previously. It is not known, however, whether l-arginine improves the situation and what's the mechanism. Leydig cells were isolated and cultured with control, 10 nM T-2 toxin, 0.25, 0.5 or 1.0 mM l-arginine, and 10 nM T-2 toxin supplemented with 0.25, 0.5 or 1.0 mM l-arginine for 24 h. Cells and supernatants were collected to detect the mRNA expression and activities of P450scc (cholesterol side-chain cleavage enzyme), 3β-HSD-1 (3β-hydroxysteroid dehydrogenase/isomerase-1) and StAR (steroidogenic acute regulatory protein). Results revealed that l-arginine increased the testosterone levels declined by T-2 toxin and up-regulated the activities and mRNA expression of P450scc, 3β-HSD-1 and StAR down-regulated by T-2 toxin in Leydig cells. Therefore, we concluded that l-arginine ameliorated the testosterone levels decreased by T-2 Toxin via regulating the mRNA expression and activities of P450scc, 3β-HSD-1 and StAR in mouse Leydig cells.

Keywords

L-arginine,Mouse Leydig cells,T-2 toxin,Testosterone,mRNA and activities,

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