Real time detection of glutathionse in chemotherapy squamous-cell carcinoma cells of a fluorescent probe.

Affiliation

Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. Electronic address: [Email]

Abstract

Glutathione (GSH) plays a key role in protecting damage induced by radiation and chemotherapy drugs. Current methods, which quantify GSH level changes by using cellular extraction or being based on redox homeostasis results, are unable to directly measure real time GSH level in live cells. In this article, we utilized a newly designed reversible fluorescent probe to measure GSH in living cells. The image of probe 1 can reveal intracellular GSH location and GSH level changes caused by different chemotherapy drugs in squamous-cell carcinoma cells. The results show that it is the first report with probe 1 that to quantify real-time GSH level changes in the cultured chemotherapy cancer cells and different changes in GSH level that may confer chemotherapy resistance.

Keywords

Chemotherapy resistance,Fluorescent probe,GSH,Real time,Squamous-cell carcinoma,