In this work, a novel facile closed bipolar electrochemiluminescence (C-BP-ECL) sensor has been developed for highly sensitive detection of glucose based on the integration of chitosan (CS), poly(diallyldimethylammonium chloride)-functioned multi-walled carbon nanotubes (PDDA-MWCNTs) and graphene quantum dots-gold nanoparticles (GQDs-AuNPs) on the wax/carbon ink-screen-printed cloth-based device. When CS, PDDA-MWCNTs and GQDs-AuNPs are successively decorated onto the cathode of closed bipolar electrode (C-BPE), the C-BPE anode can emit much stronger C-BP-ECL signals. Moreover, the cathodic decoration of the C-BPE can generate a stronger ECL signal in comparison with its anodic decoration. Under optimized conditions, glucose can be detected in the range of 0.1-5000 μM, and the limit of detection is estimated to be 64 nM, which is about three orders of magnitude lower than that in case of the bare C-BPE cathode (31 μM). It has been shown that the proposed sensor has high detection sensitivity, wide dynamic range, and as well acceptable reproducibility, selectivity and stability. Finally, the applicability and validity of the C-BP-ECL sensor are demonstrated for the detection of glucose in human serum samples. We believe that this novel highly-sensitive sensor will have potential applications in various areas such as clinical diagnosis, food analysis and environmental monitoring.