Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves.

Affiliation

Henderson BD(1), Kinahan DJ(2)(3)(4)(5), Rio J(1), Mishra R(6), King D(6), Torres-Delgado SM(7), Mager D(7), Korvink JG(7), Ducrée J(1).
Author information:
(1)School of Physical Sciences, Dublin City University, Dublin 9, Ireland.
(2)School of Mechanical Engineering, Dublin City University, Dublin 9, Ireland.
(3)National Center for Sensor Research, Dublin City University, Dublin 9, Ireland.
(4)I-Form, the SFI Research Centre for Advanced Manufacturing, Dublin City University, Dublin 9, Ireland.
(5)The Water Institute, Dublin City University, Dublin 9, Ireland.
(6)Fraunhofer Project Center, Dublin City University, Dublin 9, Ireland.
(7)Institute of Microstructure Technology, Karlsruhe Institute of Technology, 76344 Karlsruhe, Germany.

Abstract

Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL-1 and limit of quantitation (LoQ) of 135.87 ng mL-1.