Synthesis of unsymmetric perylenediimide dye molecule and its photochemical properties on lipid membrane.


The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan; Institute for Advanced Co-Creation Studies, Osaka University, 1-1 Yamadagaoka, Suita, Osaka 565-0871, Japan. Electronic address: [Email]


Optical manipulation of cellular function is one of the important targets in chemical biology and medicine. To achieve manipulation of cellular function using small molecules, photochemical reaction, such as photo-isomerization and photo-induced electron transfer, is one of the most promising reactions. Especially, photo-induced electron transfer process may be the crucial for their further development of photo-functional agents in living cells. However, such molecules, which enable the modification of cellular function, are limited and the further development is necessary. Herein, we synthesized a novel unsymmetric perylenediimide dye and investigated the cellular staining upon the addition in the cell culture medium. Furthermore, we observed the fluorescence quenching upon the addition of ascorbic acid as electron donor and report the preliminary results to manipulate Ca2+ concentration in living cell line upon 488-nm light photoirradiation.


Cellular staining,Dye,Perylenediimide,Photochemistry,