TSH inhibits eNOS expression in HMEC-1 cells through the TSHR/PI3K/AKT signaling pathway.

Affiliation

Department of Endocrinology 1, Affiliated Hospital of Jining Medical University, 272029 Jining, Shandong, China. Electronic address: [Email]

Abstract

OBJECTIVE : To investigate the effects of thyroid-stimulating hormone (TSH) on the expression of endothelial nitric oxide synthase (eNOS) in human microvascular endothelial cells (HMEC-1) and explore the potential mechanism.
METHODS : Expression of thyroid-stimulating hormone receptor (TSHR) in HMEC-1 cells was determined by immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting. Cell proliferation and the production of nitric oxide (NO) and superoxide anion (SA) were measured after TSH treatment. eNOS expression and AKT phosphorylation were detected by Western blotting.
RESULTS : TSHR was expressed in HMEC-1 cells. TSH promoted HMEC-1 cell proliferation and SA production, but inhibited NO generation by dose-dependent blocking of mRNA and protein expression of eNOS. Mechanism studies demonstrated that TSH promoted AKT phosphorylation (P<0.05), and that LY294002 inhibited the reduction of eNOS expression by TSH. Moreover, TSH activated the AKT signaling pathway through binding to TSHR on HMEC-1 cells.
CONCLUSIONS : TSH inhibits NO production via the TSHR/AKT signaling pathway.

Keywords

Cellules endothéliales microvasculaires humaines (HMEC),Endothelial nitric oxide synthase,Human microvascular endothelial cells,Nitric oxide,Oxyde nitrique (NO),Oxyde nitrique synthase endothélial (eNOS),TSHR/AKT signaling pathway,Thyroid-stimulating hormone,Thyréostimuline (TSH),Voie de signalisation TSHR/AKT,

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