Conservation of the three-dimensional aspect of bitemarks is important to optimize their correct analysis. Bitemarks could be recorded with a surface scanner. In clinical dentistry, optical cameras are used as an alternative to conventional dental impressions. They scan the dental relief for prosthetic design. We propose a three-dimensional bitemark analysis protocol using an intraoral scanner. Eight volunteers bit on different materials: dental wax and hard cheese. Then, bitemarks and dentitions were scanned with the intraoral scanner. The scans obtained were exported into the mesh comparison software, CloudCompare®, which can automatically generate overlays. For this purpose, dentition were aligned and compared with indentations. The meshing and non-meshing areas were easily visualized using a colorimetric scale. This protocol made it possible to exclude incorrect dentitions in about twenty minutes. Moreover, dentitions corresponding to bitemarks all matched. In order to refine this protocol, it must be tested with morphologically close dentitions and on the skin as well.