Ultrasensitive immunoassay for detection of zearalenone in agro-products using enzyme and antibody co-embedded zeolitic imidazolate framework as labels.

Wu X

Liu Z(1), Wang X(2), Dong F(3), Li Y(4), Guo Y(2), Liu X(4), Xu J(4), Wu X(4), Zheng Y(5).
Author information:
(1)State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Institute of Environment and Ecology, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
(2)Institute of Environment and Ecology, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
(3)State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: [Email]
(4)State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
(5)State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: [Email]

https://dx.doi.org/10.1016/j.jhazmat.2021.125276

Zearalenone (ZEN) has a potential hazard to human health, and is frequently found in agro-products. To minimize ZEN exposure to consumers, a novel metal-organic framework-based immunoassay system using zeolitic imidazolate framework-encapsulated horseradish peroxidase and goat anti-mouse IgG (HRP/Ab@ZIF-L) as labels was proposed for rapid and ultrasensitive detection of ZEN in agro-products. The HRP/Ab@ZIF-L not only maintained recognition ability of antibody and catalytic activity of enzyme, but also protected encapsulated proteins against high temperature, organic solvents and long term storage. Under optimal conditions, the detection limit of HRP/Ab@ZIF-L-based immunoassay reached 0.5 ng/L for ZEN, which was approximately 126-fold lower than that of conventional HRP-based immunoassay. Moreover, the proposed method showed an excellent selectivity, and a good dynamic linear detection for ZEN in the range of 0.5 ng/L to 0.476 μg/L. The recoveries of ZEN from spiked corn and wheat samples ranged from 84.50% to 96.70% with the relative standard deviation under 8.9%. In brief, the proposed immunoassay method has potential application for rapid and ultrasensitive detection of ZEN in agro-products.