Upregulated circular RNA circ_0074027 promotes glioblastoma cell growth and invasion by regulating miR-518a-5p/IL17RD signaling pathway.

Affiliation

Department of Immunology, Qiqihar Medical University, Qiqihar, 161006, China. Electronic address: [Email]

Abstract

Glioblastoma (GBM) is one of the major cause of the cancer-related fatality worldwide. Several circular RNAs (circRNAs) have been observed to exert functions in GBM. The current study is aimed to explore the potential mechanism of circ_0074027 via miR-518a-5p and IL17RD in GBM progression. Circ_0074027 expression was determined in a cohort of 50 pairs of GBM specimens and five cell lines by qRT-PCR. In addition, the association between circ_0074027 expression and its clinical value was analyzed by Fisher's exact test. Cell growth, clone formation, apoptosis, migration and invasion was evaluated after overexpress or knockdown the expression of circ_0074027 in GBM cells. Dual luciferase reporter assays were conducted to evaluate the relevant intermolecular target relationships. Circ_0074027 expression was evidently upregulated in GBM tissue specimens and cells compared to the adjacent non-tumorous tissues and NHA, respectively. The upregulation of circ_0074027 is related to clinical severity and exerts oncogenic functions in GBM. Moreover, circ_0074027 could sponge miR-518a-5p to release its suppression on IL17RD. Our findings provide evidence that circ_0074027 plays an oncogenic role in GBM by regulating miR-518a-5p/IL17RD signaling.

Keywords

Circular RNA,Glioblastoma,IL17RD,circ_0074027,miR-518a-5p,

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