Journal of Plant Science

ISSN: 2573-7988

Impact Factor: 0.583


Page No: 28-35

Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region



Article Reviewed By:

Elisabeth Zeukoo Menkem, PhD(

Thierry Cresteil(


LAKHDARI Wassima, Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region(2017)SDRP Journal of Plant Science 2(1):28-35


Background: This work deals with the in vitro study of the antifungal effect of aqueous extracts of six medicinal plants (Zygophyllum album, Euphorbia guyoniana, Atriplex halimus, Oudneya africana, Matricaria pubescens et Moltkiopsis ciliata) in Wadi Righ region, at their inhibition of the mycelial growth of a tomato telluric fungus (Fusarium oxysporum f. sp. radicis lycopersici).

Methods: The study of the antifungal activity of natural extracts was carried out according to the poisoned Food technique defined by Grover and Moore (1962).

Results: The in vitro study of the antifungal properties shows that all the aqueous extracts of plant species tested inhibit the mycelial growth of this phytopathogenic agent except that of Moltkiopsis ciliata. In fact, the aqueous extract of Z. album was found to be a very inhibitory solution because it induced an inhibition rate of 75.29% against the Fusarium wilt of tomato after 8 days.

Conclusion: the results indicate that the aqueous extracts studied can be considered as a very promising agent for the plant protection industry.

Key words: antifungal, aqueous extracts, Forl., medicinal plants.


  1. Dor? T, Le Bail M, Verger P. 2002. Pratiques agricoles et s?curit? sanitaire des aliments en production v?g?tale. Cahiers Agricultures, 11(13) :177-185.

  2. Ozbay N, Newman E. 2004. Biological control with Trichoderma spp. with emphasis on T. harzianum. Pakistan Journal of Biological Sciences. 7(4): 478-484.

    View Article           
  3. Diatta CD, Gueye M, Akpo L. 2013. L?onard Elie Les plantes m?dicinales utilis?es contre les dermatoses dans la pharmacop?e Ba?nounk de Djibonker, r?gion de Ziguinchor (S?n?gal). Journal of Applied Biosciences, 70:5599? 5607.

    View Article           
  4. Ould El hadj MD, Hadj-Mahammed M, Zabeirou H. 2003. Place of the spontaneous plants samples in the traditional pharmacopoeia of the area of Ouargla (Septentrional east Sahara). Courrier du Savoir-N?03, Univ. Biskra,47-51.13.

  5. Hadjadj S, Bayoussef Z, Ould El Hadj-Khelil A, Beggat H, Hammami R. 2009. Approches biochimiques et bioinformatiques pourl'?tude de la relation structure/fonction despeptides antimicrobiens d'origine v?g?tale. Th?se Doctorat. Univ. Tunis El Manar et Lille, 175 p.

  6. Lakhdari W, Dehliz A, Acheuk F, Mlik R, Hammi H, Doumandji-Mitiche B. et al. 2016. Ethnobotanical study of some plants used in traditional medicine in the region of Oued Righ (Algerian Sahara). Journal of Medicinal Plants Studies, 4(2): 204-211.

  7. CHEHMA A., 2006 - Catalogue of spontaneous plants in the northern of Algerian Sahara. Ain M'lila, Algeria, Dar Elhouda ?ditions, 2006.

  8. KHERRAZE M.H., LAKHDARI K., KHERFI Y., BENZAOUI T., BERROUSSI S., BOUHANNA M., 2014 - Atlas floristique de la vall?e de l'Oued Righ par ?cosyst?me. Touggourt, Alg?rie : Centre de recherche scientifique et technique sur les r?gions arides, 90 p.

  9. DJELLOULI M., MOUSSAOUI A., BENMEHDI H., ZIANE L., BELABBES A., BADRAOUI M., SLIMANI N. and HAMID N., 2013- Ethnopharmacological study and phytochemical screening of three plants (Asteraceae family) from the region of south west Algeria., Asian journal of natural & applied sciences.,2(2):160-165.

  10. MNAFGUI K., HAMDEN K., BEN SALAH H., KCHAOU M., NASRI M., SLAMA S., 2012 -Inhibitory Activities of Zygophyllum album: A Natural Weight-Lowering Plant on KeyEnzymes in High-Fat Diet-Fed Rats. Evidence-Based Complementary and Alternative Medicine, 12:9.

  11. Sasanelli N, Divitto M. 1991. The effect of Tagetes spp. extracts on the hatching of an Italian population of Globodera rostochiensis. Nematol.Medit., 19: 135-137.

  12. Grover RK, Moore JD. 1962. Toxicometric studies of fungicides against brown rot organisms Sclerotinia fructicola and S. laxa. Phytopathology, 52: 876- 880.

  13. Hmouni A, Oihabi L, Badoc A, Douira A. 2003. Etude de la r?sistance de Botrytis cinerea aux benzimidazoles,dicarboximides et dithiocarbamates dans les cultures abrit?es de tomate de la r?gion du Gharb (Maroc). Bull. Soc. Pharm. Bordeaux, 142 : 79-100.

  14. Cahagnier B, Richard-Molard D. 1998. Moisissures des aliments peu-hydrat?s, les moisissures. Collection sciences et techniques agroalimentaires. Ed. Lavoisier. Pp. 39-41.

  15. Sparg SG, Light ME, Van Staden J. 2004. Biological activities and distribution of plant saponins, journal of ethno pharmacology. 94 : 219- 243.

  16. Hani M, El Gamal A, Shaker KH, Karl Pollmannand KS. 1995. Triterpenoidsaponins from Zygophyllum species. Phytochemistry 40: 1233-1236. 00436-B

    View Article           
  17. El-Monayeri MO, Youssef MM, El-Ghamry N. 1981. Contributions to the autocology oftwo Zygophyllum species growing in the Egyptian desert.Journal Botanic 24;49-68.

  18. Lakhdari W, Dehliz A, Acheuk F, Soud A, Hammi H, Mlik R. 2015. Acaricidal Activity of Aqueous Extracts against the mite of date palm Oligonychus afrasiaticus Meg (Acari: Tetranychidae). Journal of Medicinal Plants Studies, 3(6): 113-117.

  19. Kemassi A, Boukhari K, Cherif R, Ghada K, Bendaken N, Bouziane N. et al. 2015. Evaluation de l'effet larvicide de l'extrait aqueux d'Euphorbiaguyoniana (Boiss. &Reut.) (Euphorbiaceae). Revue El-Wahat pour les Recherches et les Etudes, 8 (1) : 44-61.

  20. Hunsa P, Chulabhorn M, Ruchirawat S, Prawat U, Tooptakong U, Taylor W. et al. C., 1995. White cyanogenic and non-cyanogenic glycosides from Manihotesculenta. Phytochemistry., 40 (4): 1167-1173. 00398-Q

    View Article           
  21. Tripathi RD. Tiwari KP., 1980. Geniculatin, a triterpenoidsaponin from Euphorbia geniculata. Phytochemistry. 19(10): 2163-2166 . 82215-3

    View Article           
  22. Abd-ellatif S, Abdel Rahman S M, Sahar Deraz F. 2011. Promising antifungal effect of some folkloric medicinal plants collected from el- Hammam habitat, Egypt against dangerous pathogenic and toxinogenic fungi. Journal of Agricultural and Biological Science 6 (9): 25-32.

  23. Chikhi I. 2014. Composition chimique et activit?s biologiques des extraits de cinq plantes aromatiques et m?dicinales de l'ouest d'Alg?rie.Th?se Doctorat. Univ. Tlemcen, 174 p.

  24. Bouhadjera K, Kebir T, Baba-Ahmed A, Bendahou M. 2005. Antimicrobial activity of the sterols and steroids extracted from the Algerian Oudneya Africana R. Br. Pakistan J. Biol. Sci. 8:834-838.

    View Article           
  25. Ladjel S, Gherraf N, Zellagui A, Brahim L, Hameurlaine S. 2011. Phytochemical and antibacterial screening of some algerian saharian medicinal plants.Plant Sciences Feed, 1 (10): 179-178.

  26. Hammami R, Hamida JB, Vergoten G, Lacroix JM, Slomianny MC, Mohamed N, Fliss I. 2009. A new antimicrobial peptide isolated from Oudneya africana seeds. Microbiol Immunol 53(12):658-66. PMid:19954453

    View Article      PubMed/NCBI     
  27. Makhloufi A. 2009. Etude des activit?s antimicrobienne et antioxydante de deux plantes m?dicinales poussant ? l'?tat spontan? dans la r?gion de Bechar(Matricaria pubescens (Desf.) et RosmarinusofficinalisL.)et leur impact sur la conservation des dattes et du beurre cru. Th?se Doctorat. Univ. Tlemcen, 166 p.

  28. Aldoweriej AM, Alharbi KB, Saeed EMA, El-Ashmawy IM. 2016. Antimicrobial activity of various extracts from some plants native to Alqassim Region, Saudi Arabia.Journal of Food, Agriculture & Environment, 14 (1) : 14-19.

  29. Papageorgiou N, Arvanitidis C, Eleftheriou A. 2006. Multicausal environmental severity: a flexible framework for microtidal sandy beaches and the role of polychaetes as an indicator taxon. Estuar. Coast.Shelf Sci. 70: 643-653.

    View Article           
  30. Williams RM, Finefield JM, Sherman DH, Kreitman M. 2012. Enantiomeric Natural Products: Occurrence and Biogenesis. Angew. Chem. Int. Ed., 51: 4802-4836. PMid:22555867 PMCid:PMC3498912

    View Article      PubMed/NCBI     
  31. Ndukwe KC, Okeke IN, Lamikanra A, Adesina SK, Aboderin O. 2005. Antibacterial Activity of aqueous extract of selected chewing sticks. Journal of Contemporary Dental Practice. 3(6):86-94.

  32. Billerbeck VG, Roques C, Vaniere P, Marquier P. 2002. Activit? antibact?rienne et antifongique de produits ? base d'huiles essentielles. Hygiene 10(3): 248- 254.

Journal Recent Articles